Product Type

Product Information

| Features

• Contains an enzyme derived from marine bacteria with low thermal stability, allowing easy heat inactivation

• Efficiently hydrolyzes uracil-glycosidic bonds in both single-stranded and double-stranded DNA

• Effectively prevents carry-over contamination caused by successive PCR amplifications

| Results

Efficiency of uracil DNA glycosylase using PCR product (including uracil base)

Lane 1 : 10⁸ copies of a 1KB PCR product with dUTP
Lane 2 : 10⁷ copies of a 1KB PCR product with dUTP
Lane 3 : 10⁶ copies of a 1KB PCR product with dUTP
Lane 4 : 10⁵ copies of a 1KB PCR product with dUTP
Lane 5 : 100bp DNA ladder
Lane 6 : 10⁸ copies of a PCR product with dUTP
Lane 7 : 10⁷ copies of a PCR product with dUTP
Lane 8 : 10⁶ copies of a PCR product with dUTP
Lane 9 : 10⁵ copies of a PCR product with dUTP

Efficiency test of uracil DNA glycosylase was operated using serial diluted PCR products including uracil base. HiSense™ Taq Master Mix was also tested for negative control. Reaction mixture was incubated at 25°C for 5 min followed by 95°C for 5 min, 25 cycles of 30 sec at 95°C, 30 sec at 60°C, 1 min at 72°C. HiSense™ Taq Master Mix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.