CellSafe

HiSense™ QGreenBlue RT-qPCR Master Mix

Catalog No : RTQBLR-01, RTQBLR-05, RTQBHR-01, RTQBLR-05

This product is optimized for SYBR Green dye–based real-time PCR and enables consecutive reverse transcription and qPCR reactions by simply adding the RNA template and primers.

Product Type

Type Cat. No. Size
Low ROX RTQBLR-01 1 mL
  RTQBLR-05 5 mL
High ROX RTQBHR-01 1 mL
  RTQBHR-05 5 mL

 

| Cat. No. for Instruments (Depending on Rox concentration) 

Cat. No. Brand Model
(No ROX)
RTQBLR
BioRad iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384
MJ Research Opticon, Option2, Chromo4, MiniOpticon
Qiagen Roto-Gene Q, Roto-Gene3000, Roto-Gene 6000
Eppendorf Mastercycler realplex
Illumina Eco RealTime PCR System
Roche LightCycler 480, LightCycler 2.0
(High ROX)
RTQBHR
ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus
(Low ROX)
RTQBLR
ABI 7500, 7500 Fast, QuantStudio (1, 3, 5, 6, 7)
Stratagene MX3000, MX3005P, MX4000

 

Product Information

| Features

  • Enables sequential reverse transcription and qPCR reactions in a single tube by simply adding the RNA template and primers

  • Uses SYBR Green–type dye that binds to double-stranded (ds) DNA, allowing real-time fluorescence measurement for qPCR analysis

  • Hot-start PCR technology for highly specific amplification

  • Incorporates MMLV reverse transcriptase and DNA-free Taq DNA polymerase

  • Blue dye included for easy visual confirmation of the master mix

| Results

Amplification plot & Standard curve

Amplification of GAPDH gene (217bp fragment) of human cDNA

 

Specificity of HiSense™ QGreenBlue RT-qPCR Master Mix