CellSafe

HiSense™ Taq PCR Polymerase/ Master Mix/ Premix

Catalog No : DFT-500, DFT-1000, DFTM-5, LTP-96, LTP-480

This product contains a DNA polymerase derived from Thermus aquaticus.

Product Type

Type Cat. No. Size
Polymerase DFT-500 500 U
  DFT-1000 1,000 U
Master Mix DFTM-5 5 mL
Premix LTP-96 96 Tests
  LTP-480 480 Tests

Product Information

| Features

  • Source: Thermus aquaticus

  • 5′ → 3′ exonuclease activity: Yes

  • 3′ → 5′ exonuclease activity: No

  • Amplification size: < 3 kb

  • A-tailing activity: Yes

| Applications

  • Routine PCR

  • Allele-specific PCR

  • 16S and 23S rRNA gene amplification

  • Detection of bacteria in various samples (e.g., blood)

  • DNA labeling reactions and TA cloning

  • Sequencing / cycle sequencing

| Results

The amplification efficiency of HiSense™ Taq Master Mix

The cycling conditions for HiSense™ Taq PCR Master Mix  were 95°C for 5 min, 25 cycles of 95°C for 30 sec, 60°C for 30 sec, and 72°C for 1 min.

Lane 1 : 100bp DNA ladder

Lane 2,3 : 10ng human genomic DNA

Lane 4,5 : 2ng human genomic DNA

Lane 6,7 : 0.4ng human genomic DNA

Lane 8,9 : 0.08ng human genomic DNA