CellSafe

BioMycoX® Mycoplasma PCR Detection Premix

Catalog No : LD-48

This is a lyophilized product designed to detect mycoplasma contamination in research cell lines using conventional PCR. The reagents are lyophilized in 8-strip tubes, allowing users to perform the assay conveniently by simply adding the sample.

Product Type

Cat. No. Size
LD-48 48 Tests

Product Information

| Features

  • Screening of mycoplasma contamination in research cell lines

  • High-sensitivity detection of 209 mycoplasma species

  • Template DNA preparation by boiling culture supernatant or extracting genomic DNA

  • Easy detection: Supplied as a premix lyophilized in 8-strip tubes, allowing convenient assays by simply adding samples

  • High sensitivity: 10–100 copies/reaction

  • High specificity: No amplification from eukaryotic DNA or DNA from other bacterial species

  • UDG system applied: Prevents carry-over contamination

| Results

Gel Electrophoresis Result
Tested with Quantitative M. arginini Genomic DNA (Cat. No. QAGD, CellSafe)

Lane 1: 104 copies
Lane 2: 103 copies
Lane 3: 102 copies
Lane 4: 101 copies
Lane 5: 1 copy
Lane 6: Positive Control
Lane 7,8: Negative Control
Lane 9: 100bp ladder

 

  • When the PCR reaction is performed correctly, an internal control DNA band of approximately 700 bp is observed.

  • When mycoplasma contamination is present, a mycoplasma-specific DNA band of approximately 250–300 bp is detected.