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HiSense™ DLRTaq PCR Polymerase/ Master Mix/ Premix

Catalog No : DLR-500, DFDLM-5, LDLP-96, LDLP-480

40 kb 길이까지의 DNA template를 증폭하는데 최적화된 제품입니다. 첨가된 dUTPase는 고온에서 생성되는 dUTP를 제거하여 큰 사이즈의 DNA를 효과적으로 증폭하게 합니다.
제품문의

제품종류

Type Cat. No 규격
Polymerase DLR-500 500 U
Master Mix DFDLM-5 5 ml
Premix LDLP-96 96 Tests
LDLP-480 480 Tests

제품정보

| 특징

  • 5’ → 3’ exonuclease activity : Yes
  • 3’ → 5’ exonuclease activity : Yes (weak)
  • Amplification size : < 40 kb
  • A-tailing : Yes

| 적용

  • long-range amplification from genomic DNA
  • High amplification efficiency
  • Excellent performance on difficult templates
  • Amplification of low-copy targets
  • High yield and high sensitivity PCR

| 결과

The amplification efficiency of HiSense™ Taq Master Mix 
: The cycling conditions for HiSense™ Taq PCR Master Mix  were 95°C for 5 min, 25 cycles of 95°C for 30 sec, 55°C for 30 sec, and 72°C for 1 min. 

Lane 1 : 1kb plus DNA ladder
Lane 2,3 : 1kb fragment
Lane 4,5 : 3.8kb fragment
Lane 6,7 : 6.2kb fragment
Lane 8,9 : 7.5kb fragment