CellSafe

HiSense™ Taq PCR Polymerase/ Master Mix/ Premix

Catalog No : DFT-500, DFT-1000, DFTM-5, LTP-96, LTP-480

Thermus aquaticus에서 유래한 DNA Polymerase가 포함된 제품입니다.

제품종류

Type Cat. No 규격
Polymerase DFT-500 500 U
DFT-1000 1000 U
Master Mix DFTM-5 5 ml
Premix LTP-96 96 Tests
LTP-480 480 Tests

제품정보

| 특징

  • Source : Thermus aquaticus
  • 5’ → 3’ exonuclease activity : Yes
  • 3’ → 5’ exonuclease activity : No
  • Amplification size : < 3 kb
  • A-tailing : Yes

적용

  • Routine PCR
  • Allele specific PCR
  • 16S and 23S rRNA gene amplification
  • Detection of bacteria in samples (e.g. blood)
  • DNA labeling reactions & TA-cloning
  • Sequencing/ cycle sequencing

결과

The amplification efficiency of HiSense™ Taq Master Mix

The cycling conditions for HiSense™ Taq PCR Master Mix  were 95°C for 5 min, 25 cycles of 95°C for 30 sec, 60°C for 30 sec, and 72°C for 1 min.

Lane 1 : 100bp DNA ladder

Lane 2,3 : 10ng human genomic DNA

Lane 4,5 : 2ng human genomic DNA

Lane 6,7 : 0.4ng human genomic DNA

Lane 8,9 : 0.08ng human genomic DNA