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HiSense™ HotTaq PCR Polymerase/ Master Mix/ Premix

Catalog No : DFH-500, DFHM-5, LHP-96, LHP-480

Taq DNA Polymerase와 Anti-Taq 항체가 혼합된 제품으로 실온에서의 Taq DNA polymerase 활성을 억제하고, 50°C 이상에서 활성을 회복하도록 고안된 제품입니다.
제품문의

제품종류

Type Cat. No 규격
Polymerase DFH-500 500 U
Master Mix DFHM-5 5 ml
Premix LHP-96 96 Tests
LHP-480 480 Tests

제품정보

| 특징

  • Source : Thermus aquaticus
  • 5’ → 3’ exonuclease activity : Yes
  • 3’ → 5’ exonuclease activity : No
  • Amplification size : < 3 kb
  • A-tailing : Yes
  • Hot start : Yes

| 적용

  • SNP/Genotyping
  • 16S and 23S rRNA gene amplification
  • Detection of bacteria in samples (e.g. blood)
  • DNA labeling reactions & TA-cloning
  • Primer dimer 최소화

| 결과 

Specificity comparison between HiSense™ HotTaq Master Mix and HiSense™ Taq Master Mix
The cycling conditions were 25°C 1hour, 95°C for 5 min, 30 cycles of 95°C for 10 sec,  65°C for 10 sec and  72°C for 30 sec. The Anti-Taq antibody of HiSense™  HotTaq PCR Polymerase prevents the formation of misprimed products and primer-dimers at low temperatures (room temperature).

Lane 1 : 100bp DNA ladder

Lane 2,3 : 1ng human genomic DNA (HotTaq)
Lane 4,5 : 0.1ng human genomic DNA (HotTaq)

Lane 6,7 : 1ng human genomic DNA (Taq)
Lane 8,9 : 0.1ng human genomic DNA (Taq)