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TaqMan™ Universal Master Mix II, with UNG

Catalog No : 4440038

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
제품문의

 

카탈로그 번호 수량
4440038 1 x 5 mL
4440042 1 x 1 mL
4440044 2 x 5 mL
4440045 5 x 5 mL
4440046 10 x 5 mL
4440039 1 x 50 mL

 

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer components


TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

사양
Fidelity (Taq 대비) 2 X
용도(장비) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
패시브 참조 염료 ROX (Pre-mixed)
중합효소 AmpliTaq Gold DNA Polymerase
제품라인 TaqMan
제품 유형 Universal Master Mix II
순도 또는 품질 등급 UP (Ultra Pure)
수량 1 x 5 mL
샘플 종류 DNA (Genomic), cDNA
배송 조건 Wet Ice
부피 5 mL
농도 2X
검출 방법 Primer-probe
용도(애플리케이션) Gene Expression, Genotyping, miRNA analysis
GC-Rich PCR Performance High
PCR 방법 qPCR
반응 속도 Standard
Unit Size Each

 

구성 및 보관

Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).