CellSafe

BioMycoX® Mycoplasma PCR Detection Kit

Catalog No : D-25, D-50, D-100

This product is designed to detect mycoplasma contamination in research cell lines using conventional PCR.

Product Type

Cat. No. Size
D-25 25 Tests
D-50 50 Tests
D-100 100 Tests

Product Information

| Features

  • Screening of mycoplasma contamination in research cell lines

  • High-sensitivity detection of 209 mycoplasma species

  • Template DNA preparation by boiling culture supernatant or by genomic DNA extraction

  • High sensitivity: 10–100 copies/reaction

  • High specificity: No amplification from eukaryotic DNA or DNA from other bacterial species

  • UDG system applied: Prevention of carry-over contamination

| Results

Gel Electrophoresis ResultTested with Quantitative M. arginini Genomic DNA (Cat. No. QAGD, CellSafe)

Lane 1: 100bp ladder
Lane 2: 104 copies
Lane 3: 103 copies
Lane 4: 102 copies
Lane 5: 10copies
Lane 6: 1 copy
Lane 7: Positive Control
Lane 8: Negative Control

 

  • When the PCR reaction is performed correctly, an internal control DNA band of approximately 700 bp is observed.

  • When mycoplasma contamination is present, a mycoplasma-specific DNA band of approximately 250–300 bp is detected.