CellSafe

HiSense™ Mycoplasma PCR Detection Kit

Catalog No : HD-100, HDEP-100

This product enables rapid detection of mycoplasma contamination in production cell lines and rapid mycoplasma testing of biopharmaceutical products using conventional PCR.

Product Type

Cat. No. Size
HD-100 100 Tests
HDEP-100 100 Tests

Product Information

| Features

  • For mycoplasma testing (absence test) of production cell lines and biopharmaceutical products
    (Compliant with Korea MFDS, European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP) guidelines)

  • High-sensitivity detection of 209 mycoplasma species

  • Template DNA preparation by boiling culture supernatant or by genomic DNA extraction

  • High sensitivity: 1–10 copies/reaction

  • High specificity: No amplification from eukaryotic DNA or DNA from other bacterial species

  • UDG system applied: Prevention of carry-over contamination

| Results

Gel Electrophoresis Result
Tested with Quantitative M. arginini Genomic DNA (Cat. No. QAGD, CellSafe)

Lane 1: 104 copies
Lane 2: 103 copies
Lane 3: 102 copies
Lane 4: 101 copies
Lane 5: 1 copy
Lane 6: Positive Control
Lane 7,8: Negative Control
Lane 9: 100bp ladder

 

  • When the PCR reaction is performed correctly, an internal control DNA band of approximately 700 bp is observed.

  • When mycoplasma contamination is present, a mycoplasma-specific DNA band of approximately 250–300 bp is detected.