CellSafe

HiSense™ HotTaq PCR Polymerase/ Master Mix/ Premix

Catalog No : DFH-500, DFHM-5, LHP-96, LHP-480

This product is a mixture of Taq DNA polymerase and an anti-Taq antibody, designed to inhibit Taq DNA polymerase activity at room temperature and restore activity at temperatures above 50 °C.

Product Type

Type Cat. No. Size
Polymerase DFH-500 500 U
Master Mix DFHM-5 5 mL
Premix LHP-96 96 Tests
  LHP-480 480 Tests

Product Information

| Features

  • Source: Thermus aquaticus

  • 5′ → 3′ exonuclease activity: Yes

  • 3′ → 5′ exonuclease activity: No

  • Amplification size: < 3 kb

  • A-tailing activity: Yes

  • Hot start: Yes

| Applications

  • SNP / genotyping

  • 16S and 23S rRNA gene amplification

  • Detection of bacteria in various samples (e.g., blood)

  • DNA labeling reactions and TA cloning

  • Minimization of primer-dimer formation

| Results

Specificity comparison between HiSense™ HotTaq Master Mix and HiSense™ Taq Master Mix
The cycling conditions were 25°C 1hour, 95°C for 5 min, 30 cycles of 95°C for 10 sec,  65°C for 10 sec and  72°C for 30 sec. The Anti-Taq antibody of HiSense™  HotTaq PCR Polymerase prevents the formation of misprimed products and primer-dimers at low temperatures (room temperature).

Lane 1 : 100bp DNA ladder

Lane 2,3 : 1ng human genomic DNA (HotTaq)
Lane 4,5 : 0.1ng human genomic DNA (HotTaq)

Lane 6,7 : 1ng human genomic DNA (Taq)
Lane 8,9 : 0.1ng human genomic DNA (Taq)